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KMID : 1098420040120020129
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Korean Journal of Medicinal Crop Science
2004 Volume.12 No. 2 p.129 ~ p.134
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Optimal Culture Conditions for in vitro Propagation of Orostachys japonicus and Enhancement of Polysaccharide Production
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KIm Won-Jung
Kang Young-Min
Park Dong-Jin
Huh Gyung-Hye
Lee Byung-Hyun
Choi Myung-Suk
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Abstract
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Optimal culture conditions for efficient in vitro propagation and polysaccharide production of Orostachys japonicus were established. The highest growth yield was achieved in 1/2 MS medium, while the lowest growth yield was obtained in 4 MS medium. The patterns of polysaccharide formation were a little similar in all cases, but on MB5 medium, the po]ysaccharide contents of plant were higher than others. Among the modified nitrate levels, effective growth level were obtained in 1/4 N and 1/2 N. High contents of polysaccharide were obtained in 4 N. Different concentration of potassium and calcium did not improve the growth and polysaccharide production. The micropropagated shoots were successfully acclimatized artificial soils.
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KEYWORD
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Orostachys japonicus, polysaccharides, regeneration, macro nutrient
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